Logo UHasselt

menu

BIOMED


Research

BIOMED

Logo UHasselt Universiteit Hasselt - Knowledge in action

BIOIMAGING

Program Management Team

M. Ameloot, J. Hendrix, I. Lambrichts, J.-M. Rigo , B. Brône

Coordinator: Marcel Ameloot

 

 

 

 

 

 

General

The bioimaging domain aims to contribute to the recognition of BIOMED at the scientific and innovation level through advanced imaging methods and protocols offered to the other research domains. The aimed recognition in the field of imaging microscopy, especially through the label-free approach and the tracking of nanoparticles, should allow to play a role in the current open innovation model of the pharmaceutical industry.

Furthermore, our expertise in bioimaging is used to broaden and strengthen the (inter)national contacts with other academic institutions, but also with the industry.

 

Specific research focus

ex vivo 3D-fluorescence microscopy of organ slices and tissues

Develop in vivo 3D-fluorescence microscopy of parts of the brain of small animals

Utilize and improve label-free 3D-microscopy
-
based on the autofluorescence of cells, tissues and organs without exogenous labels or transfection with fluorescent proteins
- based on nonlinear optical processes in cells, tissues and organs.
- specific for stem cell research, tissue engineering and regenerative medicine.
- for the characterization of the cellular localization and dynamic behavior of nanoparticles within the context of biomarkers, nanomedicine and toxicology
 
Combine fluorescence microscopy imaging methods with electrophysiological approaches in ex vivo measurements on living brain slices

Retrieve sub-cellular (3D)-localization of specific molecules / biomarkers / receptors with cryo-TEM and 3D-EM tomography

 

 

 

Research Image of the month:

Every month, BIOMED researchers can join the contest 'research image of the month' by sending their nicest microscopy image to the BIOMED jury.

This month, the image of Jirka Cops was chosen as the microscopy highlight of the month.

"High resolution imaging of a rat tumor to evaluate apoptosis induced by intraperitoneal chemotherapy"
 

 
A cryosection of a rat omentum majus tumor with the cell nucleus in blue and activated caspase-3 in orange recorded with structured illumination microscopy on the ELYRA PS.1 (9 µm grid size, left widefield image, right processed SIM image)

 

Archive

Image of the mont of the previous months

"Cell communication by uptake of small extracellular vesicles"
(Sören Kuypers)
 
Airyscan image of HUVEC cells in red (WGA 594 cell staining) and extracellular vesicles in yellow (DiD lipid staining) recorded on the LSM880 microscope to show uptake of individual vesicles with different sizes
 
 
"Biodegradable Nanoparticles for Drug Delivery"
 (Martijn Peters)
 
The image represents HeLa cells exposed to biodegradable polymer nanoparticles with encapsulated fluorescent dye with the cell nucleus in blue, tubulin in yellow and Nanoparticles in pink
 
 
"New label-free way to visualize soot from airpolution in human lung cells"
 (Hannelore Bové)

 
A human lung cell with soot from airpolution with tubulin in purple, vimentin in yellow and white light generated by soot particles in blue.
 
 
"Rat kidney glomeruli"
(Jirka Cops)
 

 
A kidney glomeruli of a rat imaged with a Zeiss mirax desk scanner to investigate the effect of a heart condition on kidney function. (Masson’s trichrome staining method)
 
 
"Mitochondria, the power houses of the cell"
(Hannelore Bové)
 

A live cell eGFP staining of mitochondria visualized with the LSM 880. 
 
 
"Microbubbles #Bubble trouble for scientists"
(Eli Slenders)
 

 Microbubbles stained with the membrane-probe Laurdan (orange) measured with the Mai Tai laser on the Zeiss LSM510 to study the lipid organization in the shell through the photoselection effect and the local emission spectrum. More info in Slenders et al., ChemComm, 2018, 54 (38), 4854-4857.