During the last decades, the number of researchers from different disciplines performing
transcriptome analysis has increased dramatically. Despite the use of state-of-the-art highthroughput
technologies for whole genome transcriptome profiling (e.g. next generation
sequencing or microarray), quantitative real-time PCR (qPCR) remains the gold standard for
targeted gene expression analysis due to its superior sensitivity, specificity, linear dynamic range of
quantification and a high level of flexibility.
A qPCR experiment involves several steps ranging from experimental design, RNA extraction, assay
design, data generation to data analysis. While the practical performance of a real-time PCR
quantification experiment is relatively straightforward, it is important to identify critical steps that
may impact the quality and robustness of the experiment. Moreover, many researchers
experience a genuine need for more in-depth training of their experiment design and dataanalysis
skills in order to generate biologically relevant and reliable results.
To accommodate these needs, Biogazelle offers a qPCR-focused course in which the fundamental
and advanced principles of experiment design, sample and assay quality control and data analysis
- PhD students - 20 places available
- May 7 and 8, from 9:00 until 17:00
- Participation on both days is required. A sandwich lunch will be provided.
- UHasselt - campus Diepenbeek, building D, room A106
- Please bring your laptop to this course. If you do not have a laptop, please contact Stefanie Kerkhofs (email@example.com)
- Registration is closed since March 29, 2018.
- DS M&LS: category Scientfic skills - one compulsory elective technical/methods courses
- DS S&T: category Seminars & advanced courses - one advanced course