During the last decades, the number of researchers from different disciplines performing
transcriptome analysis has increased dramatically. Despite the use of state-of-the-art highthroughput
technologies for whole genome transcriptome profiling (e.g. next generation
sequencing or microarray), quantitative real-time PCR (qPCR) remains the gold standard for
targeted gene expression analysis due to its superior sensitivity, specificity, linear dynamic range of
quantification and a high level of flexibility.

A qPCR experiment involves several steps ranging from experimental design, RNA extraction, assay
design, data generation to data analysis. While the practical performance of a real-time PCR
quantification experiment is relatively straightforward, it is important to identify critical steps that
may impact the quality and robustness of the experiment. Moreover, many researchers
experience a genuine need for more in-depth training of their experiment design and dataanalysis
skills in order to generate biologically relevant and reliable results.

To accommodate these needs, Biogazelle offers a qPCR-focused course in which the fundamental
and advanced principles of experiment design, sample and assay quality control and data analysis
are covered.

For whom?

• PhD students - 20 places available

When?

• May 7 and 8, from 9:00 until 17:00
• Participation on both days is required. A sandwich lunch will be provided.

Where?

• UHasselt - campus Diepenbeek, building D, room A106

Details?

• Please bring your laptop to this course. If you do not have a laptop, please contact Stefanie Kerkhofs (stefanie.kerkhofs@uhasselt.be)

Registration?

• Registration is closed since March 29, 2018.

 Acknowledged as?

• DS M&LS: category Scientfic skills - one compulsory elective technical/methods courses
• DS S&T: category Seminars & advanced courses - one advanced course