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Doctoral School for Health & Life Sciences


Doctoral training

Doctoral School for Health & Life Sciences

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QPCR

During the last decades, the number of researchers from different disciplines performing
transcriptome analysis has increased dramatically. Despite the use of state-of-the-art highthroughput technologies for whole genome transcriptome profiling (e.g. next generation
sequencing or microarray), quantitative real-time PCR (qPCR) remains the gold standard for
targeted gene expression analysis due to its superior sensitivity, specificity, linear dynamic range of quantification and a high level of flexibility.

A qPCR experiment involves several steps ranging from experimental design, RNA extraction, assay design, data generation to data analysis. While the practical performance of a real-time PCR quantification experiment is relatively straightforward, it is important to identify critical steps that may impact the quality and robustness of the experiment. Moreover, many researchers experience a genuine need for more in-depth training of their experiment design and dataanalysis skills in order to generate biologically relevant and reliable results.

To accommodate these needs, Biogazelle offers a qPCR-focused course in which the fundamental and advanced principles of experiment design, sample and assay quality control and data analysis are covered.

For whom?

  • PhD students - 20 places available

When?

  • May 20 and 21, from 9:00 until 17:00
  • Participation on both days is required. A sandwich lunch will be provided.

Where?

  • UHasselt - campus Diepenbeek, building D, room A6.

Details?

Registration?

  • Register here until April 26, 2019.

 Acknowledged as?

  • DS M&LS: category Scientfic skills - one compulsory elective technical/methods courses
  • DS S&T: category Seminars & advanced courses - one advanced course