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GEL PERMEATION CHROMATOGRAPHY

 

TSP HPLC equipped with refactometer (Shodex)
TSP HPLC equipped with refractometer (Wyatt), MALLS(Wyatt) and Viscosimeter (Viscotek)

GPC is a separation technique which allows separating soluble polymers, resins and high molecular weight compounds on their difference in hydrodynamic volume.  A solution of the polymer is introduced under high pressure on a column containing a porous stationary phase (e.g. polystyrene-divinylbenzene) from which larger molecules will elute first.  Detection occurs with a refractometer or a UV detector.  The resulting GPC chromatogram allows to determining the molecular weight (MW) distribution after calibration with polystyrene, polyethyleneglycol or polymethacrylates.  Important parameters are Mn- , Mw-  and polydispersity (Pd). Solvents which can be used as eluens are THF, DMSO and DMF.
Triple GPC uses three sequential detectors: refractometer, viscosimeter and light scattering (MALLS) which allows determining also the morphology of polymer molecules (bar, coil) together with a more absolute MW determination.


Typical applications

  • Determination of the molecular weight distribution of soluble polymers, resins, adhesives, silicon oils.