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Imaging interactions with fluorescence: from the nano-to-macro scale


Imaging interactions with fluorescence: from the nano-to-macro scale

Logo UHasselt Universiteit Hasselt - Knowledge in action

PROGRAM

Date: September 5-7, 2018

PROGRAM AT-A-GLANCE

  Wednesday September 5 Thursday September 6 Friday September 7
Morning   09:00 am - NANO lectures 1 09:00 am - MACRO lectures 1
11:00 am - coffee break 10:45 am - coffee break
11:15 am - NANO lectures 2 11:00 am - MACRO lectures 2
Noon 1:00 pm - registration 12:45 pm - lunch 12:15 pm - group picture + lunch
Afternoon 2:00 pm - welcome 01:30 pm - MICRO exercise 1  01:30 pm - microscopy demos
2:15 pm - MICRO lectures 1                - NANO exercise 1               
3:30 pm - coffee break 03:30 pm - microscopy demos 03:30 pm - MICRO exercise 2
4:00 pm - MICRO lectures 2                  - NANO exercise 2
Evening 5:30 pm - opening mixer 07:00 pm - conference dinner in Hasselt 05:30 pm - closing words 

Lectures - Building D, auditorium H4
Lunch/Coffee breaks/receptions - Building D, Agora (front side at the windows)
Hands-on sessions - Building C (BIOMED), Basement
Pc-analyses - Units AB LL 3 & LL 4

DETAILED PROGRAM
Click here for the abstract book

Wednesday September 5

Timing Topic
2:00 pm Welcome words (Jelle Hendrix, Hasselt University)
  MICRO Lectures 1: interactions on the microscopic scale
2:15-3:00 pm Basics of fluorescence and fluorescence microscopy (Marcel Ameloot, Hasselt University)
3:00 - 3:45 pm Quantitative colocalization analyses in optical microscopy (Winnok de Vos, University of Antwerp)
3:45-4:00 pm Coffee break
  MICRO Lectures 2: interactions on the microscopic scale
4:00-4:45 pm Correlation analysis: Particles that move together also fluctuate together (Nick Smisdom, Hasselt University)
4:45-5:30 pm Fluorescence lifetimes as a tool for monitoring interactions: Principles and application to protein interactions inside retroviral particles (Jelle Hendrix, Hasselt University)
5:30-7:00 pm Opening mixer

 

Thursday September 6

Timing Topic
  NANO Lectures 1: interactions on the nanoscopic scale
9:00-9:45 am Do’s and don’t in structured illumination microscopy, and application in hepatocyte imaging (Marcel Müller & Viola Mönkemöller, KU Leuven)
9:45-10:15 am Image scanning microscopy: juggling with pixels for lateral resolution enhancement (Eli Slenders, Hasselt University)
10:15-11:00 am Superresolution optical fluctuation imaging to visualize cellular biosensors at high spatial resolution (Sam Duwé, KU Leuven)
11:00-11:15 am Coffee break
  NANO Lectures 2: interactions on the nanoscopic scale
11:15-12:00 am Single-molecule Localization Microscopy: a powerful tool to study molecular organization (Susana Rocha, KU Leuven)
12:00-12:45 am The Leica STED microscope: realtime superresolution confocal microscope (Marco Meijering, Leica Microsystems GmbH)
12:30-1:30 pm Lunch
1:30-3:30 pm Pc-analyses 1&2
MICRO exercise 1: FCS and RICS analysis in PAM
NANO exercise 1: SOFI analysis in Localize
3:30-6:30 pm Hands-on sessions 1&2
MICRO Demo 1: assessing the quality & resolution of a confocal microscope (Zeiss LSM510)
NANO Demo: PALM/STORM/SIM (Zeiss Elyra PS.1)
MICRO demo 2: studying molecular motion and interactions via FCS/RICS/FLIM (Zeiss LSM880)
MACRO demo: whole-organism light-sheet microscopy (Zeiss LightSheet Z.1)
7:00 pm Conference dinner in Hasselt

 

Friday September 7

Timing Topic
  MACRO Lectures 1: interactions on the macroscopic scale
9:00-9:45 am Light Sheet Fluorescence Microscopy- Fast and Deep Tissue Imaging (Cicerone Tudor, Zeiss Microscopy GmbH)
9:45-10:45 am Development and biomedical applications of the OpenSpinMicroscopy project for light sheet microscopy, optical projection tomography and beyond (Emilio Gualda, ICFO Spain and IGC Portugal)
10:45-11:00 am Coffee break & Legolish demo
  MACRO Lectures 2: interactions on the macroscopic scale
11:00-11:30 am Staging of tau pathology in the intact mouse brain using light sheet microscopy (Winnok de Vos, University of Antwerp)
11:30-12:15 am Imaging cellular interactions and activity in the intestinal wall using linear and non-linear optical techniques (Pieter Vanden Berghe, KU Leuven)
12:15-12:30 am Group picture
12:30-1:30 pm Lunch
1:30-3:30 pm   Hands-on sessions 3&4
MICRO Demo 1: assessing the quality & resolution of a confocal microscope (Zeiss LSM510)
NANO Demo: PALM/STORM/SIM (Zeiss Elyra PS.1)
MICRO demo 2: studying molecular motion and interactions via FCS/RICS/FLIM (Zeiss LSM880)
MACRO demo: whole-organism light-sheet microscopy (Zeiss LightSheet Z.1)
3:30-5:30 pm   Pc-analyses 3&4
MICRO exercise 2: lifetime, FLIM and phasor analysis in PAM
NANO exercise 2: SIM analysis with fairSIM
5:30-6:30 pm Closing words