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Advanced Optical Microscopy Centre

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Advanced Optical Microscopy Centre

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CD36-mediated uptake of myelin debris by macrophages and microglia reduces neuroinflammation.

Grajchen E, Wouters E, van de Haterd B, et al.
J Neuroinflammation. 2020;17(1):224. doi:10.1186/s12974-020-01899-x

Zeiss LSM880
multicolor - confocal 

Monitoring indoor exposure to combustion-derived particles using plants.

Witters K, Plusquin M, Slenders E, et al.
Environmental Pollution. 2020;266:115261. doi:10.1016/j.envpol.2020.115261

Zeiss LSM880 and Zeiss LSM510
NLO - 2PE - spectral detection - label-free

Microglial derived extracellular vesicles activate autophagy and mediate multi‐target signaling to maintain cellular homeostasis.

Van den Broek B, Pintelon I, Hamad I, et al.
J Extracell Vesicles. 2020;10(1). doi:10.1002/jev2.12022

Zeiss LSM880
multicolor - confocal

Nano-positioning and tubuline conformation determine transport of mitochondria along microtubules.

Van Steenbergen V, Lavoie-Cardinal F, Kazwiny Y, et al.
bioRxiv. 2020. doi:10.1101/2020.04.27.064766

Zeiss Elyra PS.1
live-cell - TIRF

Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the brain.

Bogie JFJ, Grajchen E, Wouters E, et al.
J Exp Med. 2020;217(5). doi:10.1084/jem.20191660

Zeiss LSM880
3D - multicolor - confocal

Fluorescence microscopy data for quantitative mobility and interaction analysis of proteins in living cells.

Lemmens V, Ramanathan K, Hendrix J.
Data in Brief. 2020;29:105348. doi:10.1016/j.dib.2020.105348

Zeiss LSM880
live-cell - RICS - spectral detection - confocal

Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles.

Merckx G, Hosseinkhani B, Kuypers S, et al.
Cells. 2020;9(2):312. doi:10.3390/cells9020312

Zeiss LSM880
multicolor - confocal

(Sub)populations of extracellular vesicles released by TNF-α –triggered human endothelial cells promote vascular inflammation and monocyte migration. 

Hosseinkhani B, van den Akker NMS, Molin DGM, Michiels L.
Journal of Extracellular Vesicles. 2020;9(1):1801153. doi:10.1080/20013078.2020.1801153

Zeiss LSM880

Live Cell Imaging Demonstrates Multiple Routes Toward a STAT1 Gain-of-Function Phenotype. 

Giovannozzi S, Lemmens V, Hendrix J, Gijsbers R, Schrijvers R.
Front Immunol. 2020;11. doi:10.3389/fimmu.2020.01114

Zeiss LSM880
live-cell - RICS - confocal

Dental Tissue and Stem Cells Revisited: New Insights From the Expression of Fibroblast Activation Protein-Alpha. 

Driesen RB, Hilkens P, Smisdom N, et al.
Front Cell Dev Biol. 2020;7. doi:10.3389/fcell.2019.00389

Zeiss LSM880
NLO - 2PE - SHG - label-free - confocal - multicolor

Raster Image Correlation Spectroscopy Performance Evaluation.

Longfils M, Smisdom N, Ameloot M, et al.
Biophysical Journal. 2019;117(10):1900-1914. doi:10.1016/j.bpj.2019.09.045

Zeiss LSM880
live-cell - RICS - confocal

Ambient black carbon particles reach the fetal side of human placenta.

Bové H, Bongaerts E, Slenders E, et al.
Nature Communications. 2019;10(1):3866. doi:10.1038/s41467-019-11654-3

Zeiss LSM880 and Zeiss LSM510
NLO - 2PE - SHG - spectral detection - label-free

Role of nanoparticle size and sialic acids in the distinct time-evolution profiles of nanoparticle uptake in hematopoietic progenitor cells and monocytes.

Wathiong B, Deville S, Jacobs A, et al.
Journal of Nanobiotechnology. 2019;17(1):62. doi:10.1186/s12951-019-0495-x

Zeiss LSM880
3D - multicolor - Airyscan - 2PE

Effect of Branching on the Optical Properties of Poly(p-phenylene ethynylene) Conjugated Polymer Nanoparticles for Bioimaging.

Braeken Y, Cheruku S, Seneca S, et al.
ACS Biomater Sci Eng. 2019;5(4):1967-1977. doi:10.1021/acsbiomaterials.8b01416

Zeiss LSM880
live-cell - 2PE - NLO

Body surface area-based versus concentration-based intraperitoneal perioperative chemotherapy in a rat model of colorectal peritoneal surface malignancy: pharmacologic guidance towards standardization.

Lemoine L, Thijssen E, Carleer R, et al.
Oncotarget. 2019;10(14):1407-1424. doi:10.18632/oncotarget.26667

Zeiss Elyra PS.1
multicolor - widefield - SIM

Fluorescent PCDTBT Nanoparticles with Tunable Size for Versatile Bioimaging. Materials.

Cheruku S, D’Olieslaeger L, Smisdom N, et al.
Materials. 2019;12(15):2497. doi:10.3390/ma12152497

Zeiss LSM880 and Zeiss LSM510
live-cell - RICS - 2PE - NLO - confocal

SlZRT2 Encodes a ZIP Family Zn Transporter With Dual Localization in the Ectomycorrhizal Fungus Suillus luteus.

Coninx L, Smisdom N, Kohler A, et al.
Front Microbiol. 2019;10. doi:10.3389/fmicb.2019.02251

Zeiss LSM880 and Zeiss Elyra PS.1
live-cell - confocal - widefield - multicolor

Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells.

Slenders E, Bové H, Urbain M, et al.
J Phys Chem Lett. 2018;9(20):6112-6118. doi:10.1021/acs.jpclett.8b02686

Zeiss LSM880 and Zeiss LSM510
ICS - NLO - SHG - 2PE - labelfree

Crosstalk-free multicolor RICS using spectral weighting.

Schrimpf W, Lemmens V, Smisdom N, Ameloot M, Lamb DC, Hendrix J.
Methods. 2018;140-141:97-111. doi:10.1016/j.ymeth.2018.01.022

Zeiss LSM880
live-cell - RICS - spectral detection - confocal