Zeiss LSM510 meta

Use this highly versatile confocal microscope to solve multi-dimensional research questions. Equipped with an NLO module for deep-tissue and label-free imaging, a spectral detector, and two PMTs for confocal fluorescence microscopy, the LSM510 provides a convenient solution for most of your imaging needs.

Picture of a Zeiss AxioVert 100M microscope stand equipped with a dark PECON incubator. An LSM510 confocal microscope scanhead is attached to the left side port. Picture of a Zeiss AxioVert 100M microscope stand equipped with a dark PECON incubator. An LSM510 confocal microscope scanhead is attached to the left side port.

Use and training

To access this microscope, contact the facility to schedule a hands-on training session. Trained users have 24/7 access to the microscope and are free to schedule experiments using the online booking system. Training can only be given by facility staff.

Acknowledgements

Thank you for acknowledging our imaging facility. You can use the statement below in all your publications that include data acquired on the Zeiss LSM510:

We acknowledge the Advanced Optical Microscopy Centre at Hasselt University for support with the microscopy experiments.

Specifications

Excitation

  • HBO100 fluorescence lamp
  • CW Argon Laser with 458, 477, 488 and 514nm lines
  • CW HeNe 543nm laser
  • CW HeNe 633nm laser
  • Pulsed MaiTai-HPDS laser (690-1040nm)

Filter sets

The microscope and scanhead contain a large amount of filter turrets and exchangeable filtercube positions. The following table provides an overview of the standard filter configuration of the LSM880. In addition to these filters, the spectral detection unit (Quasar) allows filter free selection of the detection wavelength range.

Filter overview

Reflector revolver

Main beam spitter

Secondary beam splitter 1

Secondary beam splitter 2

Secondary beam splitter 3

NDD beam splitter

Emission filter Ch1

Emission filters Ch2

Position

None

NT 80/20

None

Mirror

None

HFT 488

KP 695

LP 560

1

FSet01 wf (blue)

HFT UV/488/543/633

Mirror

NFT 490

Plate

FT 605

BP 390-465 IR

BP 510/20

2

FSet09 wf (green)

HFT KP 700/488

NFT 490

NFT 545

BG 39

FT 442

BP 435-485 IR

BP 500-550 IR

3

FSet15 wf (red)

HFT KP 700/543

NFT 515

BG 39

Mirror

HBO Mirror

BP 480-520 IR

BP 535-590 IR

4

NDD KP685

HFT 458/514

NFT 545

BP 500-530 IR

BP 565-615 IR

5

HFT 458

NFT 635 VIS

BP 500-550 IR

None

6

HFT 488

NFT KP 545

LP 505

BP 650-710 IR

7

HFT KP 650

Plate

LP 505

8

Detection

The microscope is equipped with the following detectors:

  • Meta scanning module with 2 single channel detectors and a 32 channel spectral detector.
  • T-PMT transmission detector.
  • NDD detection module with 2 NDD detectors.

Objective lenses

The microscope is equipped with a variety of low- and high-magnification and NA objectives:

Objective lens overview

Objective lens

Free working distance

Max resolution *

Immersion

Remarks

Plan-Neofluar 10x/0.30

5.2 mm

1.07 x 11.67 µm

None (air)

Zeiss information page 

EC Plan Neofluar 20x/0.50

2.0 mm

0.64 x 4.20 µm

None (air)

Zeiss information page 

Plan ApoChromat 20x/0.75

0.6 mm

0.43 x 1.87 µm

None (air)

Zeiss information page

LD C-ApoChromat 40x/1.10 W Korr UV-VIS-IR

0.62 mm

0.29 x 0.87 µm

Water

Zeiss information page 

EC Plan Neofluar 40x/1.30 Oil DIC

0.21 mm

0.25 x 0.62 µm

Oil

Zeiss information page 

Alpha Plan ApoChromat 100x/1.46

0.11 mm

0.22 x 0.49 µm

Oil

Zeiss information page 

* Theoretically obtainable lateral (xy) by axial (z) resolution according to the Rayleigh resolution criterium at an emission wavelength of 525 nm.
Lateral resolution (xy) = 0.61 * wavelength / numerical aperture
Axial resolution (z) = 2 * wavelength / (numerical aperture)²

Other features

A PECON incubation system allowing precise control of experimental conditions (Temperature and CO2).

Applications

The LSM510 is a very versatile setup, which finds its use in diverse applications, including:

  • live deep-tissue two-photon fluorescence microscopy (e.g. migrating cells in live tissue slices).
  • labelfree imaging (e.g. SHG imaging of the extracellular matrix).
  • multi-dimensional confocal imaging (xyztc).
  • ...

Performance

Laserpower stability:

Recorded laserpower when requesting 1% transmission. Laserpowers are recorded on the microscope stage with the objective lens removed.

[laserpower graphs]

Laserpower linearity:

Recorded laserpower at various requested % transmission. Laserpowers are recorded on the microscope stage with the objective lens removed. Results of the most recent laserpower measurements are shown in a log-log plot.

[laserpower linearity graph]

Contact

Sam Duwé - AOMC

Location
Agoralaan, Building C, 3590 Diepenbeek
Function
Microscopy expert