Brône - Rigo Neurophysiology lab (1)

"Excited about inhibition & moved by migration"

Prof. dr. Jean-Michel Rigo

Neurodevelopment
Electrophysiology
Ligand-gated ion channels
Microglia
Docimologie


Contact:
+32 (11) 26 80 04
jeanmichel.rigo@uhasselt.be 


Research

Our brain is an intriguing organ that gradually becomes more active and refined during its development.
It is not surprising that disruption of brain development through genetic and environmental factors leads to neurodevelopmental diseases such as autism spectrum disorder and schizophrenia.

Since proper neurodevelopment is driven by the complex interplay between neurons and microglia, we focus on the impact of genetic and environmental factors on these cells. A genetic risk factor for autism spectrum disorder, directly acting at neuronal networks, is the impaired glycine receptor signaling in the brain.

Environmental factors impact the highly dynamic immune cells of the brain: microglia. Using molecular, cellular and behavioral approaches, we aim to understand the normal and pathological brain development in order to identify new targets with therapeutic potential  for neurodevelopmental disorders.

People

Prof. dr. Bert Brône
bert.brone@uhasselt.be

Prof. dr. Jean-Michel Rigo
jeanmichel.rigo@uhasselt.be


Microglia research line

dr. Yeranddy A. Alpizar 
yeranddy.aguiaralpizar@uhasselt.be 
Project title: Molecular mechanisms of microglial dynamics


Sofie Kessels
sofie.kessels@uhasselt.be 
Project title: Microglial dynamics in neurodevelopmental disorders


Chloe Trippaers
cloe.trippaers@uhasselt.be 
Project title: Microglia and synapses in neurodevelopmental disorders


Melanie Mertens
Melanie.mertens@uhasselt.be
Project title: TRPV4 in spinal cord injury


Andreea Burlacu
andreea.burlacu@uhasselt.be
Project title: Diamond probes to study subcellular control of cytoskeletal rearrangement by temperature sensitive ion channels in microglia



Glycine receptor research line

dr. Elisabeth Piccart
elisabeth.piccart@uhasselt.be 
Project title: Glycinergic modulation of midbrain signal integration in schizophrenia


Yana Vella
yana.vella@uhasselt.be
Project title: Subcellular localisation and function of glycine receptor alpha 2


Lisa Berden
lisa.berden@uhasselt.be
Project title: A closer look at brain inhibition and seizure susceptibility following embryonic DNA damage (collaboration with SCK-CEN)


Sarra Zaghbouni
sarra.zaghbouni@uhasselt.be
Project title: Diamond probes to study subcellular control of cytoskeletal rearrangement by temperature sensitive ion channels in neurons


In collaboration:

Ben Rombaut
ben.rombaut@uhasselt.be
Project title: Microglial dynamics in neurodevelopmental disorders (co-supervisor)


Keerthana Ramanathan
keerthana.ramanathan@uhasselt.be 
Project title: Molecular mechanisms of microglial dynamics in neurodevelopmental disorders (co-supervisor)


Kinga Réka Tasnady
kingareka.tasnady@uhasselt.be
Project title: Gastrointestinal function, enteric neuron connectivity, and microbiome analysis in a mouse model for major mental illness

Techniques

  • Cell and brain slice electrophysiology,
  • Time lapse imaging in acute brain slices
  • High-end imaging techniques
  • Optogenetics

  • Migration and phagocytosis assays
  • Flow cytometry

  • Animal behavioral tests
  • Stereotactic surgery

Publications

Both authors contributed equally

Smolders SMT, Kessels S, Vangesewinkel T, Rigo JM, Legendre P and Brone B. Microglia: Brain cells on the move. Progress in Neurobiology. (2019); [IF: 10,568 (2018)].

Molchanova SM, Comhair J, Karadurmus D, Piccart E, Harvey RJ, Rigo JM, Schiffmann SN, §Brone B and §Gall D. Tonically active alpha2 subunit-containing glycine receptors regulate the excitability of striatal medium spiny neurons. Front Mol Neurosci. (2018); 10 442 [IF: 3,720].

Comhair J, Devoght J, Morelli G, Harvey RJ, Briz V, Borrie SC, Bagni C, Rigo JM, Schiffmann SN, Gall D, §Brone B and §Molchanova SM. Alpha2-containing glycine receptors promote neonatal spontaneous activity of striatal medium spiny neurons and support maturation of glutamatergic inputs. Front Mol Neurosci. (2018); 11 380 [IF: 3,720].

Smolders SM, Swinnen N, Kessels S, Arnauts K, Smolders S, Le Bras B, Rigo JM, Legendre P and Brone B. Age-specific function of alpha5beta1 integrin in microglial migration during early colonization of the developing mouse cortex. Glia. (2017); 65 (7): 1072-1088 [IF: 5,846].

Morelli G, Avila A, Ravanidis S, Aourz N, Neve RL, Smolders I, Harvey RJ, §Rigo JM, §Nguyen L and §Brone B. Cerebral cortical circuitry formation requires functional glycine receptors. Cereb Cortex. (2017); 27 (3): 1863-1877 [IF: 6,559].

In collaboration

Morelli G, Even A, Gladwyn-Ng I, Le Bail R, Shilian M, Godin JD, Peyre E, Hassan BA, Besson A, Rigo JM, Weil M, Brone B and Nguyen L. P27(kip1) modulates axonal transport by regulating alpha-tubulin acetyltransferase 1 stability. Cell Rep. (2018); 23 (8): 2429-2442 [IF: 7,815].

Even A, Morelli G, Broix L, Scaramuzzino C, Turchetto S, Gladwyn-Ng I, Le Bail R, Shilian M, Freeman S, Magiera MM, Jijumon AS, Krusy N, Malgrange B, Brone B, Dietrich P, Dragatsis I, Janke C, Saudou F, Weil M and Nguyen L. Atat1-enriched vesicles promote microtubule acetylation via axonal transport. Sci Adv. (2019); 5 (12): eaax2705 [IF: 12.804 (2018)].

Stancu IC, Cremers N, Vanrusselt H, Couturier J, Vanoosthuyse A, Kessels S, Lodder C, Brone B, Huaux F, Octave JN, Terwel D and Dewachter I. Aggregated tau activates nlrp3-asc inflammasome exacerbating exogenously seeded and non-exogenously seeded tau pathology in vivo. Acta Neuropathol. (2019); 137 (4): 599-617 [IF: 18,174 (2018)].

Torre-Muruzabal T, Devoght J, Van den Haute C, Brone B, Van der Perren A and Baekelandt V. Chronic nigral neuromodulation aggravates behavioral deficits and synaptic changes in an alpha-synuclein based rat model for parkinson's disease. Acta Neuropathol Com. (2019); 7 (1): [IF: 5.883 (2019)].

Microglia game

Play the Microglia-game!