Project R-6302

Neuroprotective and neuroregenerative effects of dental pulp stem cell transplantation in ischaemic stroke (Research)

Stroke is a severe condition defined by loss of brain function due to impaired blood flow to the brain. It is a major cause of disability and is the second leading cause of death worldwide. Modern medicine is unable to sufficiently improve the functional outcome after stroke. Currently, the only FDA-approved treatment can be used in merely 2-4% of the patients, highlighting the urgent need for improved therapy. Stem cell therapy is considered a promising approach to minimize neurological damage and enhance functional recovery after stroke. Human dental pulp stem cells (hDPSCs) are ideal candidates for stem cell therapy. These stem cells secrete high amounts of key factors for the survival and regulation of neuronal cells. Moreover, enhanced functional recovery after transplantation of hDPSCs in a rodent stroke model has been demonstrated. Our data show that hDPSCs enhance neurite outgrowth in neuronal precursor cells, which is an indication for their neuroregenerative potential. We hypothesize that hDPSCs improve the functional outcome after ischaemic stroke via neuroprotection and stimulation of neuroregeneration. The capacity of hDPSCs to exert neuroprotective and neuroregenerative effects is evaluated. Furthermore, their effects are investigated in a mouse stroke model. By conducting this study we gain insight in the therapeutic potential of hDPSCs in stroke and reveal valuable information for translation to an effective cell-based treatment of this severe condition.

01 October 2015 - 30 September 2016