Flow Cytometry Unit

BD LSRFortessa

The BD LSRFortessa is equipped with four lasers and can detect FSC, SSC and up to 16 colours simultaneously. It is coupled with a high-throughput sampler (HTS), allowing the acquisition of samples in 96-wells plates.

Cytek Aurora

The Cytek Aurora is a spectral flow cytometer, equipped with 4 lasers (V/B/YG/R) and 48 + 3 detectors, which allows for measuring the full spectrum of fluorescent signals. This technology significantly improves sensitivity, resolution, and increases the number of fluorochrome combinations that can be made. This device is also equipped with an ESP module, which allows for small particle detection, down to 70 nm (e.g. extracellular vesicles).

BD FACSAria Fusion

The BD FACSAria Fusion is equipped with four lasers and can detect FSC, SSC, and up to 16 colours simultaneously. The setup of this device is similar to that of our BD LSRFortessa, allowing for the direct translation of your high-parameter phenotyping panel into a sorting panel to isolate your subset of interest. The device is placed within a biosafety cabinet, to ensure sample quality during cell sorting.

BD Rhapsody™ HT Express System

The BD Rhapsody Express enables single cell capture for analysis of genomic and proteomic information. BD offers a range of different options for murine and human multi-omics analysis, including targeted mRNA expression analysis, whole transcriptome analysis, surface protein expression analysis (using the BD AbSeq technology), TCR/BCR sequencing, and nuclei sequencing. This latest version enables users to simultaneous run single-cell capture of 8 individual samples. Additional equipment needed to perform library preparation following single cell capture is also available at the Flow Cytometry Unit. If you are interested in setting up your own single cell multi-omics experiment, please contact the Flow Cytometry Unit at an early stage so we can help you maximize your results while minimizing your investment.

Software packages: FACSDiva v9; FlowJo v10 with additional plug-ins; FlowJo v11; OMIQ; SpectroFlo

We have expertise in every aspect of flow cytometry. Therefore we can assist you from start to finish: from panel design and optimization to ensure reliable and reproducible data, to data analysis using manual gating techniques or state-of-the-art clustering algorithms depending on your needs. 

Our dedicated expert team ensures the highest standards of research through proactive maintenance and technical troubleshooting. Beyond instrument care, we provide hands-on support for experimental design and analysis, guaranteeing precision and reliability for every project.

Examples of our areas of expertise:

• Manual gating for analysis of complex datasets (up to 18 parameters)
• Manual gating for analysis of rare immune cell subsets
• Cell proliferation and suppression analysis
• Cytometric bead array data analysis
• Clustering algorithms (e.g. FlowSOM, t-SNE, UMAP, …) 

Curiox Laminar Wash System HT2000

The Curiox Laminar Wash System is designed to perform centrifugation-free gentle washing of cells during sample preparation for flow cytometry, FACS, or single cell sequencing. This technique allows for faster and more effective washing of your cells compared to centrifugation. With this system, 96 samples can be processed simultaneously and a full wash cycle (comparable to three rounds of centrifugation) is done in three minutes. See the Curiox website for more details on the Laminar Wash system (link: https://www.curiox.com/).  

GentleMACS

The GentleMACS Octo Dissociator provides standardized, automated tissue processing to ensure optimal cell viability and reproducibility. By streamlining dissociation, homogenization, and perfusion, it delivers the high-quality single-cell suspensions essential for sensitive downstream applications like scRNAseq and multi-color flow cytometry.