Project R-11946
Title
Investigation of G-protein coupled receptors conformational
dynamics via single-molecule fluorescence microscopy. (Research)
Abstract
G-protein coupled receptors (GPCRs) orchestrate critica! processes in the human body, including
neurotransmission, neurodegeneration, vision, inflammation, pain, etc. In this project, the pipeline for systematic
studies of GPCR conformational dynamics based on advanced methods of single-molecule fluorescence
microscopy wil! be developed and applied to one of the most well-described GPCR - A2A-adenosine receptor. This
receptor is considered as a prospective target for drugs against insomnia, chronic pain, depression, and
Parkinson's disease. The first steps of the project will focus on instrumental challenges. During this phase, we
wil! use the known protein reé:overin as a model protein avoiding known membrane protein challenges. The
scheduled experiments wil! for the first time provide data on conformational dynamics of recoverin with single
molecule resolution. Further studies of conformational dynamics of calcium sensors wil! open the gates for clinical
applications in the treatment of a wide spectrum of neurological and neurodegenerative diseases from autism to
Parkinson's disease and Alzheimer's disease.
Period of project
01 September 2021 - 31 December 2023