Resources
This page contains the operating procedures for the AOMC equipment. Follow these procedures while working with the equipment to ensure optimal performance and durability. If you have any questions regarding these procedures or want to request a specific procedure, please contact AOMC staff.
Additionally, you can find a selection of interesting microscopy resources (articles, tutorials, tools, ...), and a list of publications in collaboration with AOMC.
Operating procedures, guides and manuals
Zeiss LSM880 NLO Airyscan
Zeiss LSM880 NLO Airyscan
Basic instructions: Download the SOP here.
- switching the system ON
- switching the system OFF
- cleaning procedures
Confocal experimental setup: Download the SOP here.
- mounting a sample
- locating a sample
- multi-color experiment
- z-stack experiment (3D)
- time series experiment (movies, timelapse)
- tilescan experiment (larger sample areas)
- data storage principles
Airyscan experimental setup: Download the SOP here.
- Airyscan setup
- Airyscan FAST setup
Zeiss LSM510 meta
Zeiss LSM510 meta
Coming soon.
Zeiss Elyra PS.1
Zeiss Elyra PS.1
Basic instructions: Download the SOP here.
- switching the system ON
- switching the system OFF
- cleaning procedures
Nikon Eclipse Ti2-E
Nikon Eclipse Ti2-E
Basic instructions: Download the SOP here.
- switching the system ON
- switching the system OFF
- cleaning procedures
Peripherals
Peripherals
Thorlabs S175C powermeter (high power):
Setting up the S175C high intensity powermeter sensor to measure the power of the pulsed lasers (e.g. MaiTai).
Download the S175C powermeter instructions here.
Thorlabs S170C powermeter (low power):
Setting up the S170C low intensity powermeter sensor to measure the output power of the continuous lasers (e.g. Argon or HeNe).
Interesting resources
A selection of interesting microscopy articles and reviews:
- Jonkman, J., Brown, C. M., Wright, G. D., Anderson, K. I. & North, A. J. Tutorial: guidance for quantitative confocal microscopy. Nat Protoc (2020) doi:10.1038/s41596-020-0313-9.
- Jimenez, A., Friedl, K. & Leterrier, C. About samples, giving examples: Optimized Single Molecule Localization Microscopy. Methods 174, 100–114 (2020). doi:10.1016/j.ymeth.2019.05.008.
- North, A. J. Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition. J Cell Biol 172, 9–18 (2006). doi:10.1083/jcb.200507103.
A selection of interesting microscopy resources:
- Microlist: a searchable database for light microscopy resources.
- Molecular Expressions: educative microscopy website.
- Nikon MicroscopyU: educative microscopy website.
- ZEISS Campus: educative microscopy website.
- Olympus microscopy resource center: educative microscopy website.
A selection of interesting video resources and tutorials:
- Microcourses: short videos on light microscopy topics.
- iBiology techniques: a light microscopy lectures series (also covers other topics).
- ZEN knowledge base: a collection of instruction videos on how to use Zeiss equipment and software.
A selection of interesting fluorophore and filter resources:
- FPbase: an extensive fluorescent protein database.
- Semrock Searchlight: view fluorophore and filter spectra.
- Chroma spectra-viewer: view fluorophore and filter spectra.
- ThermoFisher spectra-viewer: view fluorophore and filter spectra.