Resources

This page contains the operating procedures for the AOMC equipment. Follow these procedures while working with the equipment to ensure optimal performance and durability. If you have any questions regarding these procedures or want to request a specific procedure, please contact AOMC staff.

Additionally, you can find a selection of interesting microscopy resources (articles, tutorials, tools, ...), and a list of publications in collaboration with AOMC.

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Operating procedures, guides and manuals

Zeiss LSM880 NLO Airyscan

Basic instructions: Download the SOP here.

  • switching the system ON
  • switching the system OFF
  • cleaning procedures

Confocal experimental setup: Download the SOP here.

  • mounting a sample
  • locating a sample
  • multi-color experiment
  • z-stack experiment (3D)
  • time series experiment (movies, timelapse)
  • tilescan experiment (larger sample areas)
  • data storage principles

Airyscan experimental setup: Download the SOP here.

  • Airyscan setup
  • Airyscan FAST setup

Zeiss LSM510 meta

Coming soon.

Zeiss Elyra PS.1

Basic instructions: Download the SOP here.

  • switching the system ON
  • switching the system OFF
  • cleaning procedures

Nikon Eclipse Ti2-E

Basic instructions: Download the SOP here.

  • switching the system ON
  • switching the system OFF
  • cleaning procedures

Peripherals

Thorlabs S175C powermeter (high power):

Setting up the S175C high intensity powermeter sensor to measure the power of the pulsed lasers (e.g. MaiTai).

Download the S175C powermeter instructions here.

Thorlabs S170C powermeter (low power):

Setting up the S170C low intensity powermeter sensor to measure the output power of the continuous lasers (e.g. Argon or HeNe).

Download the S170C powermeter instructions here.

Interesting resources

A selection of interesting microscopy articles and reviews:

  • Jonkman, J., Brown, C. M., Wright, G. D., Anderson, K. I. & North, A. J. Tutorial: guidance for quantitative confocal microscopy. Nat Protoc (2020) doi:10.1038/s41596-020-0313-9.
  • Jimenez, A., Friedl, K. & Leterrier, C. About samples, giving examples: Optimized Single Molecule Localization Microscopy. Methods 174, 100–114 (2020). doi:10.1016/j.ymeth.2019.05.008.
  • North, A. J. Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition. J Cell Biol 172, 9–18 (2006). doi:10.1083/jcb.200507103.

A selection of interesting microscopy resources:

A selection of interesting video resources and tutorials:

A selection of interesting fluorophore and filter resources:

Contact

Sam Duwé - AOMC

Location
Agoralaan, Building C, 3590 Diepenbeek
Function
Microscopy expert