Resources

This page contains the operating procedures for the AOMC equipment. Follow these procedures while working with the equipment to ensure optimal performance and durability. If you have any questions regarding these procedures or want to request a specific procedure, please contact AOMC staff.

Additionally, you can find a selection of interesting microscopy resources (articles, tutorials, tools, ...), and a list of publications in collaboration with AOMC.

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Operating procedures, guides and manuals

Zeiss LSM880 NLO Airyscan

Basic instructions: Download the SOP here.

  • switching the system ON
  • switching the system OFF
  • cleaning procedures

Confocal experimental setup: Download the SOP here.

  • mounting a sample
  • locating a sample
  • multi-color experiment
  • z-stack experiment (3D)
  • time series experiment (movies, timelapse)
  • tilescan experiment (larger sample areas)
  • data storage principles

Airyscan experimental setup: Download the SOP here.

  • Airyscan setup
  • Airyscan FAST setup

Zeiss LSM900 AiryScan 2

Information coming soon.

Zeiss Lightsheet 7

Information coming soon.

Zeiss Elyra PS.1

Basic instructions: Download the SOP here.

  • switching the system ON
  • switching the system OFF
  • cleaning procedures

Nikon Eclipse Ti2-E

Basic instructions: Download the SOP here.

  • switching the system ON
  • switching the system OFF
  • cleaning procedures

Peripherals

Thorlabs S175C powermeter (high power):

Setting up the S175C high intensity powermeter sensor to measure the power of the pulsed lasers (e.g. MaiTai).

Download the S175C powermeter instructions here.

Thorlabs S170C powermeter (low power):

Setting up the S170C low intensity powermeter sensor to measure the output power of the continuous lasers (e.g. Argon or HeNe).

Download the S170C powermeter instructions here.

Interesting resources

Publications using AOMC equipment:

Performing microscopy experiments and analysis:

Reporting microscopy data acquisition and analysis:

  • Montero Llopis, P. et al. Best practices and tools for reporting reproducible fluorescence microscopy methods. Nat Methods 1–14 (2021) doi:10.1038/s41592-021-01156-w.
  • Aaron, J. & Chew, T.-L. A guide to accurate reporting in digital image processing – can anyone reproduce your quantitative analysis? J Cell Sci 134, (2021). doi: 10.1242/jcs.254151.
  • Heddleston, J. M., Aaron, J. S., Khuon, S. & Chew, T.-L. A guide to accurate reporting in digital image acquisition – can anyone replicate your microscopy data? J Cell Sci 134, (2021). doi: 10.1242/jcs.254144.

Basics of microscopy:

Video resources and tutorials:

Fluorophore and filter databases:

Contact

Sam Duwé - AOMC

Location
Agoralaan, Building C, 3590 Diepenbeek
Function
Microscopy expert