Data Management & Storage

• The UHasselt Storage Guide
• The UHasselt RDM policy

Performing microscopy experiments and analysis:

• The Quantitative BioImaging Guide
• Introduction to BioImage Analysis
• Jonkman, J., Brown, C. M., Wright, G. D., Anderson, K. I. & North, A. J. Tutorial: guidance for quantitative confocal microscopy. Nat Protoc (2020). doi:10.1038/s41596-020-0313-9.
• Jimenez, A., Friedl, K. & Leterrier, C. About samples, giving examples: Optimized Single Molecule Localization Microscopy. Methods 174, 100–114 (2020). doi:10.1016/j.ymeth.2019.05.008.
• North, A. J. Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition. J Cell Biol 172, 9–18 (2006). doi:10.1083/jcb.200507103.

Reporting microscopy data acquisition and analysis:

• Montero Llopis, P. et al. Best practices and tools for reporting reproducible fluorescence microscopy methods. Nat Methods 1–14 (2021) doi:10.1038/s41592-021-01156-w.
• Aaron, J. & Chew, T.-L. A guide to accurate reporting in digital image processing – can anyone reproduce your quantitative analysis? J Cell Sci 134, (2021). doi: 10.1242/jcs.254151.
• Heddleston, J. M., Aaron, J. S., Khuon, S. & Chew, T.-L. A guide to accurate reporting in digital image acquisition – can anyone replicate your microscopy data? J Cell Sci 134, (2021). doi: 10.1242/jcs.254144.

Basics of microscopy:

• Microlist: a searchable database for light microscopy resources.
• Molecular Expressions: educative microscopy website.
• Nikon MicroscopyU: educative microscopy website.
• ZEISS Campus: educative microscopy website.
• Olympus microscopy resource center: educative microscopy website.

Video resources and tutorials:

• Microcourses: short videos on light microscopy topics.
• iBiology techniques: a light microscopy lectures series (also covers other topics).
• ZEN knowledge base: a collection of instruction videos on how to use Zeiss equipment and software.

Fluorophore and filter databases:

• FPbase: an extensive fluorescent protein database.
• Semrock Searchlight: view fluorophore and filter spectra.
• Chroma spectra-viewer: view fluorophore and filter spectra.
• ThermoFisher spectra-viewer: view fluorophore and filter spectra.

Publications using AOMC equipment:

• The AOMC Publications spreadsheet - see below - lists (almost) all of the recent publications that made use of AOMC equipment.