NanoMacro Microscopy Workshop detailed program

2:00 pm

Welcome words (Jelle Hendrix, Hasselt University)

MICRO Lectures 1: interactions on the microscopic scale

2:15 - 3:00 pm

Basics of fluorescence and fluorescence microscopy (Marcel Ameloot, Hasselt University)

3:00 - 3:45 pm

Quantitative colocalization analyses in optical microscopy (Winnok de Vos, University of Antwerp)

3:45 - 4:00 pm

Coffee break

MICRO Lectures 2: interactions on the microscopic scale

4:00 - 4:45 pm

Correlation analysis: Particles that move together also fluctuate together (Nick Smisdom, Hasselt University)

4:45 - 5:30 pm

Fluorescence lifetimes as a tool for monitoring interactions: Principles and application to protein interactions inside retroviral particles (Jelle Hendrix, Hasselt University)

5:30 - 7:00 pm

Opening mixer

NANO Lectures 1: interactions on the nanoscopic scale

9:00 - 9:45 am

Do’s and don’t in structured illumination microscopy, and application in hepatocyte imaging (Marcel Müller & Viola Mönkemöller, KU Leuven)

9:45 - 10:15 am

Image scanning microscopy: juggling with pixels for lateral resolution enhancement (Eli Slenders, Hasselt University)

10:15 - 11:00 am

Superresolution optical fluctuation imaging to visualize cellular biosensors at high spatial resolution (Sam Duwé, KU Leuven)

11:00 - 11:15 am

Coffee break

NANO Lectures 2: interactions on the nanoscopic scale

11:15 - 12:00 pm

Single-molecule Localization Microscopy: a powerful tool to study molecular organization (Susana Rocha, KU Leuven)

12:00 - 12:45 pm

The Leica STED microscope: realtime superresolution confocal microscope (Marco Meijering, Leica Microsystems GmbH)

12:30 - 1:30 pm

Lunch

1:30 - 3:30 pm

Pc-analyses 1&2
MICRO exercise 1: FCS and RICS analysis in PAM
NANO exercise 1: SOFI analysis in Localize

3:30 - 6:30 pm

Hands-on sessions 1&2
MICRO Demo 1: assessing the quality & resolution of a confocal microscope (Zeiss LSM510)
NANO Demo: PALM/STORM/SIM (Zeiss Elyra PS.1)
MICRO demo 2: studying molecular motion and interactions via FCS/RICS/FLIM (Zeiss LSM880)
MACRO demo: whole-organism light-sheet microscopy (Zeiss LightSheet Z.1)

7:00 pm

Conference dinner in Hasselt

MACRO Lectures 1: interactions on the macroscopic scale

9:00 - 9:45 am

Light Sheet Fluorescence Microscopy- Fast and Deep Tissue Imaging (Cicerone Tudor, Zeiss Microscopy GmbH)

9:45 - 10:45 am

Development and biomedical applications of the OpenSpinMicroscopy project for light sheet microscopy, optical projection tomography and beyond (Emilio Gualda, ICFO Spain and IGC Portugal)

10:45 - 11:00 am

Coffee break & Legolish demo

MACRO Lectures 2: interactions on the macroscopic scale

11:00 - 11:30 am

Staging of tau pathology in the intact mouse brain using light sheet microscopy (Winnok de Vos, University of Antwerp)

11:30 - 12:15 pm

Imaging cellular interactions and activity in the intestinal wall using linear and non-linear optical techniques (Pieter Vanden Berghe, KU Leuven)

12:15 - 12:30 pm

Group picture

12:30 - 1:30 pm

Lunch

1:30 - 3:30 pm

Hands-on sessions 3&4
MICRO Demo 1: assessing the quality & resolution of a confocal microscope (Zeiss LSM510)
NANO Demo: PALM/STORM/SIM (Zeiss Elyra PS.1)
MICRO demo 2: studying molecular motion and interactions via FCS/RICS/FLIM (Zeiss LSM880)
MACRO demo: whole-organism light-sheet microscopy (Zeiss LightSheet Z.1)

3:30 - 5:30 pm

Pc-analyses 3&4
MICRO exercise 2: lifetime, FLIM and phasor analysis in PAM
NANO exercise 2: SIM analysis with fairSIM

5:30 - 6:30 pm

Closing words