Jeol JEM-1400Flash

Use and training

To acquire data on this microscope, please contact Esther Wolfs.

Acknowledgements

Thank you for acknowledging our imaging resources. You can use the statement below in all your publications that include data acquired on the Jeol JEM-1400Flash:

Transmission electron microscopy was made possible by the Research Foundation Flanders (FWO, grant I000220N)

Specifications

The JEM-1400Flash is a compact and user-friendly TEM, largely recognized for high-resolution imaging and analysis, as well as its ease of use. To smoothly transition from low to high magnification and acquire higher-throughput image data, the JEM-1400Flash has an integrated high-sensitivity sCMOS camera and an ultra-wide area montage system.

• LaB6 filament
• Accelerating voltage 10 - 120 kV
• Magnification x10 to x 1,200,000
• High sensitivity "Matataki Flash" sCMOS camera
  • 2,048 x 2,048 pixels
  • 21.7 x 21.7 µm² effective pixel size
  • up to 30 frames per second (unbinned)
  • 16 bit
• EMSIS XAROSA 20MP Camera
  • 5,120 x 3,840 pixels
  • 13 x 13 µm² effective pixel size
  • up to 30 frames per second (unbinned)
• Selection of holders (single, tilt +/- 70°, quartet, rotation)

Correlative Light Electron Microscopy (CLEM)

For CLEM, a digital image acquired with an optical microscope can be overlaid on a TEM image. This technique combines spatiotemporal information from a fluorescence/confocal microscope with high-resolution structural data from TEM. Hence, CLEM combines functional information with ultrastructural information to gain in-depth knowledge on a subcellular and molecular level.

• Correlative light and electron microscopy software (CLEM) installed to combine confocal images with TEM.
• Cryo Transfer holder single-tilt
• Cryo work station and temperature controller.

Sample Preparation Services

we offer sample preparation services for a wide range of TEM applications. Please contact us to discuss which methods are best suited for your project.

• Conventional TEM sample processing using resin embedding and ultrathin sectioning
• High pressure freezing & cryosubstitution for cryo-TEM as a tool for preserving high-quality ultrastructure and immunoreactivity, without prior fixation.