Zeiss Lightsheet 7
The Zeiss Lightsheet 7 microscope is perfectly suited for fast volumetric imaging of sensitive samples such as living model organisms or organoids. Additionally, the microscope can accommodate large cleared samples as a whole and image them with sub-cellular details thanks to dedicated refractive index matched optics. The dual sided illumination coupled with the pivot scan reduces illumination artefacts, two cameras allow for fast simultaneous dual color acquisitions, and the available sample chambers and sample mounting tools can accommodate almost any sample.
Use and training
To access this microscope, contact the facility to schedule a hands-on training session. Trained users have 24/7 access to the microscope and are free to schedule experiments using the online booking system. Training can only be given by facility staff.
Acknowledgements
Thank you for acknowledging our imaging facility. You can use the statement below in all your publications that include data acquired on the Zeiss LM880:
We acknowledge the Advanced Optical Microscopy Centre at Hasselt University for support with the microscopy experiments. Microscopy was made possible by the Research Foundation Flanders (FWO, Large Research Infrastructure Grant I001222N).
Specifications
Excitation
- CW Solid State 405nm Laser (50mW)
- CW Solid State 445nm Laser (25mW)
- CW Solid State 488nm Laser (50mW)
- CW Solid State 561nm Laser (50mW)
- CW Solid State 638nm Laser (75mW)
Filter sets
The microscope is equipped with a range of compatible filter sets.
Fluorophore (color) | Excitation | Beamsplitter | Emission | Common fluorophores |
|---|---|---|---|---|
Blue Green | 405 488 | SBS LP 490 | BP 420-470 BP 505-545 | DAPI GFP / Alexa Fluor 488 |
Blue Red | 405 561 | SBS LP 510 | BP 420-470 BP 575-615 | DAPI mCherry / Alexa Fluor 555 |
Cyan Red | 445 561 | SBS LP 510 | BP 460-500 LP 585 | CFP mCherry / Alexa Fluor 555 |
Green Red | 488 561 | SBS LP 560 | BP 505-545 LP 585 | GFP / Alexa Fluor 488 mCherry / Alexa Fluor 555 |
Green Far-red | 488 638 | SBS LP 560 | BP 505-545 LP 660 | GFP Alexa Fluor 647 |
Detection
The microscope is equipped with the following detectors:
- 2x pco.edge 4.2 sCMOS camera (82% QE, 6.5µm pixels)
Objective lenses
The microscope is equipped with a variety of low- and high-magnification objectives for imaging in aqueous and clearing solutions:
Objective lens overview
Objective lens | Free working distance | Max resolution * | Refractive index | Remarks |
|---|---|---|---|---|
Illumination Objectives | ||||
5x/0.1 foc | / | / | / | Zeiss information page |
10x/0.2 foc | / | / | / | Zeiss information page |
Detection Objectives | ||||
Fluar 2.5x/0.12 M27 | 8.7 mm | / | / | |
EC Plan-NEOFLUAR 5x/0,16 | 18.5 mm | / | Adaptive | |
W Plan-Apochromat 10x/0.5 | 3.7 mm | / | 1.33 | |
W Plan-Apochromat 40x/1,0 DIC | 2.1 mm | / | 1.33 | |
Clr Plan-Neofluar 20x/1.0 Corr nd=1.45 | 5.6 mm | / | 1.45 | |
Clr Plan-Neofluar 20x/1,0 Korr nd=1,53 | 6.4 mm | / | 1.53 |
* Theoretically obtainable lateral (xy) by axial (z) resolution according to the Rayleigh resolution criterium at an emission wavelength of 525 nm.
Lateral resolution (xy) = 0.61 * wavelength / numerical aperture
Axial resolution (z) = 2 * wavelength / (numerical aperture)²
Other features
- Temperature and CO2 incubation.
- Multiple sample chambers, including:
- Samples in aeqeous solutions (n=1.33).
- Samples in clearing solutions (n=1.35-1.58).
- Large sample chamber (n=1.33-1.58).
- Translucence Mesoscale chamber for extra large samples.
Applications
The Lightsheet 7 is a powerful microscope ideally equipped for volumetric imaging of medium sized samples. :
- Volumetric imaging of organoids and tissues.
- Simultaneous and sequential multi-color acquisitions.
- Fast timeseries tracking real-time events.
- Lightsheet FRET imaging.
- ...