Use this highly versatile state-of-the-art confocal microscope to solve all your multi-dimensional research questions. Equipped with sensitive GaAsP PMT detectors, flexible variable beam splitter dichroics and the latest Airyscan 2 detection system for super-resolved fluorescence microscopy, the LSM900 provides a convenient solution for most of your imaging needs. Additionally, the AI Sample Finder and advanced Navigation and Tiles module make it very easy to find regions of interest and set up your imaging experiments.
To access this microscope, contact the facility to schedule a hands-on training session. Trained users have 24/7 access to the microscope and are free to schedule experiments using the online booking system. Training can only be given by facility staff.
Thank you for acknowledging our imaging facility. You can use the statement below in all your publications that include data acquired on the Zeiss LSM900:
We acknowledge the Advanced Optical Microscopy Centre at Hasselt University for support with the microscopy experiments. Microscopy was made possible by the Research Foundation Flanders (Large Research Infrastructure Grant I001222N).
The microscope and scanhead are equipped with dedicated filters and variable beam splitter dichroics for optimal detection by the three detector units.
The microscope is equipped with the following detectors:
The microscope is equipped with a variety of low- and high-magnification and NA objectives:
Objectives listed in bold are permanently available. Other listed objective lenses can be provided on demand.
One position is permanently occupied by the AI Sample Finder deflecting mirror.
Free working distance
Max resolution *
EC Plan Neofluar 5x/0.16 M27
2.00 x 41.02 µm
Plan-ApoChromat 20x/0.80 M27
0.40 x 1.64 µm
Plan-ApoChromat 63x/1.40 Oil DIC M27
0.23 x 0.54 µm
EC Plan-Neofluar 10x/0.30 M27
1.07 x 11.67 µm
EC Plan Neofluar 20x/0.50 M27
0.64 x 4.20 µm
Plan-ApoChromat 40x/0.95 Korr M27
0.34 x 1.16 µm
LD C-ApoChromat 40x/1.10 W Korr UV-VIS-IR M27
0.29 x 0.87 µm
C-ApoChromat 63x/1.20 W Korr M27
0.27 x 0.73 µm
* Theoretically obtainable lateral (xy) by axial (z) resolution according to the Rayleigh resolution criterium at an emission wavelength of 525 nm.
Lateral resolution (xy) = 0.61 * wavelength / numerical aperture
Axial resolution (z) = 2 * wavelength / (numerical aperture)²
The LSM900 is a powerful and versatile confocal microscope capable of fulfilling all your confocal imaging needs including: