Nikon Eclipse Ti2-E with Kinetix sCMOS camera and CoolLED pE-800 LED excitation
This fully motorized microscope with an exceptional field-of-view is equipped with a powerful multicolor LED lightsource and a Photometrics Kinetix camera for very fast imaging of large sample areas, making it the perfect match for all your functional imaging needs.
Use and training
To access this microscope, contact the facility to schedule a hands-on training session. Trained users have 24/7 access to the microscope and are free to schedule experiments using the online booking system. Training can only be given by facility staff.
Acknowledgements
Thank you for acknowledging our imaging facility. You can use the statement below in all your publications that include data acquired on the Nikon Ti2-E:
We acknowledge the Advanced Optical Microscopy Centre at Hasselt University for support with the microscopy experiments. Microscopy was made possible by the Research Foundation Flanders (FWO, project G036320N), the Francqui foundation (project FRANCQBOEW) and the Dutch Research Council (NWO VIDI 016.196.367).
Specifications
Excitation
- CoolLED pE800 MB (400nm)
- 400 nm
- 435 nm
- 470 nm
- 500 nm
- 550 nm
- 580 nm
- 635 nm
- 740 nm
Filter sets
- LED-CFP/YFP/mCherry-3X-A
- LED-DAPI/FITC/TRITC/Cy5/Cy7-5X-A
Lightsource & filtercube | Excitation | Beamsplitter | Emission | Common fluorophores |
|---|---|---|---|---|
CoolLED pE800 - 400 | 414– 450 nm | DAPI | ||
CoolLED pE800 - 435 | 464 – 486 nm | CFP | ||
CoolLED pE800 - 470 | 500 – 530 nm | GFP, FITC | ||
CoolLED pE800 - 500 | 532 – 554 nm | YFP | ||
CoolLED pE800 - 550 | 580 – 611 nm | TRITC | ||
CoolLED pE800 - 580 | 603 – 800 nm | mCherry | ||
CoolLED pE800 - 635 | 661 – 701 nm | Cy5, Alexa Fluor 647 | ||
CoolLED pE800 - 740 | 769 – 850 nm | Cy7 |
Detection
The microscope is equipped with a Photometrics Kinetix back-illuminated sCMOS camera
- 500 fps
- 29.4 mm FOV diagonal
- 95% QE
- 6.5 µm x 6.5 µm pixels area
Objective lenses
The microscope is equipped with low and high magnification objective lenses:
- CFI Plan Fluor 4x/0.13
- CFI Plan-ApoChromat Lambda 20x/0.75
- CFI Plan-ApoChromat VC 60XA/1.20 WI
Other features
- Extra large FOV (25 mm) to capture more data in a single acquisition
Applications
Typical research applications of the Nikon fast widefield microscope include:
- cell-tracking of fluorescently labelled cells passed through cell culture flow chambers.
- cell migration studies.
- calcium imaging.
- ...