Use this highly versatile state-of-the-art confocal microscope to solve all your multi-dimensional research questions. Equipped with an NLO module for deep-tissue and label-free imaging, pulsed excitation and time-resolved detection for FLIM, a spectral detector, and an AiryScan detection system for super-resolved fluorescence microscopy, the LSM880 provides a convenient solution for most of your imaging needs.
To access this microscope, contact the facility to schedule a hands-on training session. Trained users have 24/7 access to the microscope and are free to schedule experiments using the online booking system. Training can only be given by facility staff.
Thank you for acknowledging our imaging facility. You can use the statement below in all your publications that include data acquired on the Zeiss LM880:
We acknowledge the Advanced Optical Microscopy Centre at Hasselt University for support with the microscopy experiments. Microscopy was made possible by the Research Foundation Flanders (FWO, project G0H3716N).
The microscope and scanhead contain a large amount of filter turrets and exchangeable filtercube positions. The following table provides an overview of the standard filter configuration of the LSM880. In addition to these filters, the spectral detection unit (Quasar) allows filter-free selection of the detection wavelength range.
MBS invisible light
MBS visible light
SBS emission filter
Airyscan emission filter
FSet43 wf (red)
FSet49 wf (blue)
SBS BP 420-460 + LP500
BP 420-460 + BP 495-550
FSet38 wf (green)
SBS LP 525
BP 420-480 + BP 495-620
SBS LP 570
BP 420-480 + LP 605
SBS LP 660
BP 465-505 + LP 525
SBS LP 460
BP 495-535 + LP 555
SBS SP 615
BP 555-620 + LP 645
The microscope is equipped with the following detectors:
The microscope is equipped with a variety of low- and high-magnification and NA objectives:
Free working distance
Max resolution *
EC Plan-Neofluar 10x/0.30 M27
1.07 x 11.67 µm
Plan-ApoChromat 20x/0.80 M27
0.40 x 1.64 µm
EC Plan Neofluar 20x/0.50 M27
0.64 x 4.20 µm
LD C-ApoChromat 40x/1.10 W Korr UV-VIS-IR M27
0.29 x 0.87 µm
C-ApoChromat 63x/1.20 W Korr M27
0.27 x 0.73 µm
Plan-ApoChromat 63x/1.40 Oil DIC M27
0.23 x 0.54 µm
Plan-ApoChromat 40x/0.95 Korr M27
0.34 x 1.16 µm
* Theoretically obtainable lateral (xy) by axial (z) resolution according to the Rayleigh resolution criterium at an emission wavelength of 525 nm.
Lateral resolution (xy) = 0.61 * wavelength / numerical aperture
Axial resolution (z) = 2 * wavelength / (numerical aperture)²
Documentation about Phasor analysis: Find more information here.
Because the LSM880 is such a versatile setup, it finds use in a large selection of diverse applications, including: