Leica LMD7 with THUNDER
This advanced Leica system offers precise laser microdissection and innovative THUNDER computational clearing for exceptional sample analysis. The LMD7's focused laser beam allows for the accurate isolation of specific cells or regions of interest from diverse biological samples. THUNDER computational clearing enhances visualization by removing out-of-focus haze, providing clear, high-contrast images of even the thickest specimens. This integrated approach ensures superior data quality for your research, delivering both precise sample preparation and detailed 3D image data.
Use and training
To access this microscope, contact the facility to schedule a hands-on training session. Trained users have 24/7 access to the microscope and are free to schedule experiments using the online booking system. Training can only be given by facility staff.
Acknowledgements
Thank you for acknowledging our imaging facility. You can use the statement below in all your publications that include data acquired on the Leica LMD7 with THUNDER:
We acknowledge the Advanced Optical Microscopy Centre at Hasselt University for support with the microscopy experiments. Microscopy was made possible by the Research Foundation Flanders (FWO I001024N).
Specifications
Excitation & Filter Sets
- CoolLED pE300 White
- DAPI ET,k - ex BP350/50, dc LP400, em BP460/50
- GFP ET,k - ex BP470/40, dc LP495, em BP525/50
- TXR ET,k - ex BP560/40, dc LP585, em BP630/75
- Y5 ET,k - ex BP620/60, dc LP660, em BP700/75
Detection
The microscope is equipped with a Leica K3C color camera:
- 3072 x 2048 pixels
- 2.4 µm x 2.4 µm pixels area
- RGB color filter (Bayer)
- up to 15 frames / sec
Additionally, the microscope is equipped with a Leica K5 cooled sCMOS camera for fluorescence readout:
- 2048 x 2048 pixels
- 80% QE
- 6.5 µm x 6.5 µm pixel area
Objective lenses
The microscope is equipped with low and high magnification objective lenses:
- UVI 5x/0.12 Microdissection
- HC PL FLUOTAR 10x/0.32 PH1
- HC PL FLUOTAR L 20x/0.40 CORR PH1
- HC PL FLUOTAR L 40x/0.60 CORR PH2 XT
Other features
- THUNDER 3D DCV Enhancement Package
- Instant computational clearing
- Small and Large volume computational clearing (3D)
- THUNDER live
Applications
Typical research applications of the Leica LMD 7 with THUNDER include:
- Microdissection of cells and tissue areas.
- Multicolor fluorescence imaging.
- High quality transmission imaging.
- ...