Miltenyi MACSima

Use and training

To access this microscope, contact the facility to schedule a hands-on training session. Trained users have 24/7 access to the microscope and are free to schedule experiments using the online booking system. Training can only be given by facility staff.

Acknowledgements

Thank you for acknowledging our imaging facility. You can use the statement below in all your publications that include data acquired on the Miltenyi MACSima:

We acknowledge the Advanced Optical Microscopy Centre at Hasselt University for support with the microscopy experiments. Microscopy was made possible by EFRO Vlaanderen (EFRO-project ‘Aging Screen’ , projectnumber 2073) and the Limburgs Kankerfonds.

Specifications

Excitation

• High-powered LED excitation:
  • 386/23 nm
  • 420/10 nm
  • 470/40 nm
  • 531/46 nm
  • 628/32 nm
  • 725/40 nm
• 2W separate bleaching unit

Emission

• • 470/40 nm
  • 530/30 nm
  • 580/25 nm
  • 698/70 nm
  • 809/81 nm

Objective lenses 

The microscope is equipped with low and high resolution objective lenses:

• 2× objective to generate overview images; NA 0.1 
• 20× long working distance objective (designed for 1 mm thick slides); NA 0.45
• 20× objective with high numerical aperture (designed for 170 μm thick cover glass); NA 0.75

Camera

• High resolution cooled sCMOS
  • 25mm field of view

Other features

• Hardware and image-based autofocus.
• Automated liquid handling system.
• High-precision automated stage for reliable positioning (accuracy 100nm).

Applications

Typical research applications of the Miltenyi MACSima include:

• High-plex spatial protein profiling.
• Biomarker studies.
• Spatial proteomics.
• ...